![]() Objetivou-se avaliar a atividade anticoagulante dos polissacarídeos sulfatados (PS) isolados de uma rodofícea do gênero Halymenia, nativa do litoral cearense, Brasil. floresia interfered on coagulation cascade.Ī crescente carência de heparina (HEP) motiva a busca por fontes alternativas de novos anticoagulantes naturais. ![]() The compound was less active than heparin, but its anticoagulant mechanism suggested that it is dependent on cofactor heparin II to inhibit thrombin activity, but not on cofactors VIII and IX. Hf2s showed a higher anticoagulant activity (68.4 IU mg -1) than those of Hf1s and Hf3s, whose activities were 37.6 and 36.6 IU mg -1, respectively. The anticoagulant properties of Hf2s containing 53.8% sulfate and 3% protein was also compared to those of heparin (193.0 IU mg -1) by assays of activated partial thromboplastin time (APTT) and thrombin time (TT) using normal human plasma. ![]() The polysaccharides are composed mainly of galactose, with small amounts of xylose and glucose. The three differential extractions yielded 38.6%. In this study, crude sulfated polysaccharide fractions (denominated Hf1, Hf2 and Hf3) were obtained from the red marine alga Halymenia floresia and the anticoagulant properties of a soluble crude polysaccharide fraction (Hf2s) were assayed. lentillifera extract may have a potential anticoagulant property due to its component SP.Īlternative sources of anticoagulants have arisen as a result of the increasing demand for safer anticoagulant clinical therapy, and the sulfated polysaccharides of seaweeds have gained attention in biomedicine. Simple linear regression revealed a positive significant correlation (multiple R = 0.9450, R² = 0.8931, P = 0.02) implying dose-dependent anticoagulant potential. lentillifera extract had the longest coagulation time (15.49 min). In phase two, all blood samples mixed with EDTA did not clot, while the negative control had an average clotting time of 2.01 min. Phase one results revealed significant differences on the clotting time between the negative and the positive and experimental groups (P 0.05). Coagulation was evaluated by measuring the clotting time using the tube method. ![]() In each collection, the sample was divided into five aliquots: (1) negative control (normal saline solution), (2) positive control (ethylenediaminetetraacetic acid ), and (3-5) experimental treatments at 0.1, 0.15, and 0.2 ml of C. In phase two, peripheral blood from three apparently healthy adult dogs were used. Blood coagulation was evaluated by measuring the clotting time using the slide and tube methods. Phase one utilized nine male albino rabbits, which were randomly and equally allocated into three groups: (1) negative control (oral distilled water and subsequent in vitro mixing of extracted blood with normal saline solution), (2) positive control (oral aspirin and subsequent in vitro mixing of extracted blood with normal saline solution), and (3) experimental group (oral distilled water and in vitro mixing of extracted blood with C. lentillifera extract was tested in two phases. lentillifera was washed thoroughly with distilled water and manually expressed to obtain the extract. The study generally aimed to evaluate the potential anticoagulant property of Caulerpa lentillifera. Sulfated polysaccharides (SP), which have anticoagulant properties, are found in most seaweeds, including Caulerpa spp. Please be careful when you join their group.Anticoagulants have been used in the treatment of several circulatory diseases and thrombotic disorders, and in the blood sampling for hematologic analysis. We are aware that there are other fake groups copying our name. Please don’t forget to join our Facebook Group – Mama’s Guide Recipes (Official Group). Thanks for dropping by our website! Do you want to be notified with our latest recipes? Just hit “yes” on the subscribe window and allow us to notify you with new recipes everyday.
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